Humulus japonicus is an annual vine plant of the family Cannabaceae, and is an ecosystem disturbing plant that grows wild throughout Korea. In this study, the effect of Humulus japonicus extract on Human hair follicle dermal papilla cell (HFDPC) was confirmed, and hair products containing Humulus japonicus extract were prepared, and the anti-hair loss efficacy of hair products containing Humulus japonicus extract was confirmed through clinical trials.