Change of Inhibitory Activity against Helicobacter pylori by Culture Condition of Morus alba cv. Cheongmoknosang Callus

Jung-Woo Chae1, Hye-Jin Park2, Sun-Ae Kang2, Bong-Jeun An3, Dong-Hyun Ahn4, Myung-Uk Kim5 and Young-Je Cho6*

1Gyeonggi-do Forest Environment Research Institute, Osan 447-290, Korea  2School of Applied Bioscience, Kyungpook National University, Daegu 702-701, Korea 3Department of Cosmeceutical Science, Daegu Hanny University, Gyeongsan, 712-715, Korea 4Department of  Food Science & Technology, Institute of Food Science, Pukyong National University, Busan 608-737, Korea 5Gyungbuk Institute for Marine Bio-Industry, Uljin 767-813, Korea 6School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University, Daegu 702-701, Korea

Inhibitory effect of useful components from Cheongmoknosang mulberry leaves extracts against Helicobacter pylori were investigated to develope them to a health functional food. For biomass application of Cheongmoknosang mulberry leaf, the optimum culture condition was tested. It was found that optimum culture condition for spot culture of Cheongmoknosang mulberry leaf was 3% sucrose at 27℃ for 20days with MS medium. Callus formation of Cheongmoknosang has an influence on naphthalene acetic acid(NAA), 2,4-dichlorophenoxy acetic acid(2,4-D),  6-benzylaminopurine(BA) and kinetin. These growth regulators including 10 μM NAA, 2,4-D, BA and kinetin in MS medium showed good growth rate of callus by incubation at 27℃ for 20 days. The growth rate of callus was higher in mixed hormone treatment than that of single hormone treatment. The optimum culture condition for Cheongmoknosang callus was 3% sucrose at 27℃ for days with 10 μM each of NAA, 2,4-D and BA mixed hormones in MS medium.