The Increase of Anti-inflammation Activity of Extracts from Saururus chinensis by Elicitor Treatment

Jung-Woo Chae1†, Hye-Jin Park6, Shin-Hyub Hong6, Ju-Yeong Lee2, Young-Mi Goo6, Jun-Hyo Cho3, Myung-Uk Kim4, Bong-Jeun An5 and Young-Je Cho6

1Gyeonggi-do Forest Environment Research Institute, Osan 447-290,  Korea

2School of Applied Bioscience, Kyungpook National University, Daegu 702-701, Korea

3Department of Environmental Technology, Kyungpook National University, Daegu 702-701, Korea

4Gyungbuk Institute for Marine Bio-Industry, Uljin 767-813, Korea

5Department of Cosmeceutical Science, Daegu Hanny University, Gyeongsan, 712-715, Korea

6School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University, Daegu 702-701, Korea

In the case of MTT assay measuring the cell survival rate, Saururus chinensis extract produced by biomass method was added at concentration range of 6.25~50μM/mL. None change was shown in cell viability at all concentration. To investigate NO product inhibition mechanism connection with iNOS, used immunblot analysis measuring iNOS protein expression inside cytoplasm by western blot. Elicitor treated Saururus chinensis extract showed iNOS product inhibition concentration dependently and elicitor treated Saururus chinensis extract 25 μM/mL concentration showed 70% iNOS product inhibition effect which is higher then that of none treated Saururus chinensis extract. Higher treatment concentration showed more excellent iNOS product inhibition effect identifying similar level to normal group. Measuring COX-2 protein expression which is another inflammatory factor when treating with LPS, elicitor treated Saururus chinensis extract inhibited COX-2 expression concentration dependently. 25 μM/mL of elicitor treated Saururus chinensis extract showed much more higher COX-2 expression inhibitory effect then none treated Saururus chinensis extract as 80% to 20%. Low concentration treatment at 6.25~12.5 μM/mL showed more excellent COX-2 expresstion inhibitory effect concentration dependently. Elicitor treated Saururus chinensis leave extract showed better expression inhibitory effect on iNOS , COX-2 and NO production then none treated Saururus chinensis extract. Elicitor treatment can be seen as appropriate method for manipulating biological active compounds of Saururus chinensis. As a result, elicitor treatment method on Saururus chinensis produced by biomass can be applied to healthy functional material or medicinal material furthermore.