Anti-oxidative Activities of Extracts from un-ripening Pinus koraiensis Siebold. et Zucc. cone

Jung-Woo Chae1, Ju-Yeong Lee1, Dong-Hee Kim2, Hyun-Sik Kim3, Su-Bin Lim3, Sung-Sook Chun3 and Young-Je Cho3*

1Gyeonggi-do Forest Environment Research Institute, Osan 447-290,  Korea.

2Korea Promotion Institute for Oriental Medicine Industry

3School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University, Daegu 702-701, Korea.

Pinus koraiensis Siebold. et Zucc. cone as a main stuff was collected in Gyeonggi-do Gapyeong county. The optimal extraction solvent of un-ripening Pinus koraiensis Siebold. et Zucc. cone were water extract and 80% ethanol. The optimal time to extract phenoilc compounds of Pinus koraiensis cone was 12 hr.  As a result, phenoilc compounds were showed 3.7±0.9 mg/mL and 4.9±0.8 mg/mL in each condition. Antioxidant activities were evaluated using DPPH radical scavenging activity, ABTS radical cation decolorization, antioxidant protection factor and TBARS. As the results, inhibitory ability of ABTS was getting high dependantly as the concentration of added phenolics increased. When phenolics of 200 μg/mL were added, inhibitory activity of un-ripening Pinus koraiensis Siebold. et Zucc. cone extract was similar with control (BHA 72.8%). β-carotene was mixed with emulsion containing linoleic acid then determined the antioxidant activity against lipophilic substances, it showed a high antioxidant activity as the protection factor (PF) was higher than control (BHA) at phenolic concentrations of 50~200 μg/mL. TBARS also inhibited the oxidation of lipophilic substances significantly like control. Therefore un-ripening Pinus koraiensis Siebold. et Zucc. cone extracts are very excellent substances for making to use industry of anti-oxidative materials.

Key words: Anti-oxidative Activities, phenolics, extracts, un-ripening Pinus koraiensis cone.