Whitening Effect of Extracts from Un-ripeing Pinus koraiensis Siebold. et Zucc. cone in B16F10 Melanoma cell

Jung-Woo Chae1, Dong-Hee Kim2, Myung-Uk Kim3, Jae-Bum Jo4, Eun-Ho Lee4, Shin-Hyup Hong4, Sung-Sook Chun4 and Young-Je Cho4*

1Gyeonggi-do Forest Environment Research Institute, Osan 447-290,  Korea.

2Korea Promotion Institute for Oriental Medicine Industry

3Gyungbuk Institute for Marine Bio-Industry, Uljin 767-813, Korea

4School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University, Daegu 702-701, Korea.

By determining tyrosinase inhibition activity in Melanoma cell (B16F10) cell, un-ripening Pinus koraiensis Siebold. et Zucc. cone water extract and ethanol extract 25μg/mL showed 25% and 30% inhibition rate each relatively low. Determining melanin biosynthesis content in Melanoma cell (B16F10), 40% melanin synthesis inhibition rate was shown at 10 μg/mL un-ripening Pinus koraiensis Siebold. et Zucc. cone ethanol extracts. Determining inhibition effect with un-ripening Pinus koraiensis Siebold. et Zucc. cone on tyrosinase expression which involve in melanin synthesis, 60% inhibition rate of tyrosinase protein expression by 10 μg/mL alcohol extract in B16F10 group. Investigated expression inhibition effect of a melanin synthesizing factor, microphthalmia associated transcription factor (MITF). At 10 μg/mL concentration, it decreased to the lowest MITF expression of 30%. Therefore, un-ripening Pinus koraiensis Siebold. et Zucc. cone extracts are very excellent substances for making to use industry of functional materials.

Key words: Whitening effect, extracts, Un-ripeing Pinus koraiensis cone, B16F10 Melanoma cell