Anti-inflammation Effect of Extracts from un-ripening Pinus koraiensis Siebold. et Zucc. cone in Raw 264.7 Cell induced Lipopolysaccharide

Jung-Woo Chae1, Ju-Yeong Lee1, Dong-Hee Kim2, Ki-Tae Park3, Jun-Hyo Cho4, Eun-Ju Choi5, Sung-Sook Chun5 and Young-Je Cho5*

1Gyeonggi-do Forest Environment Research Institute, Osan 447-290,  Korea.

2Korea Promotion Institute for Oriental Medicine Industry

3School of Culinary Art and Baking technology, Dongju College University, Busan 604-715, Korea

4Department of Environmental Technology, Kyungpook National University, Daegu 702-701, Korea

5School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University, Daegu 702-701, Korea.

Anti-inflammation effect of un-ripening Pinus koraiensis Siebold. et Zucc. cone extracts on NO, iNOS, COX-2 and cytokine was determined using cell line. It was determined that un-ripening Pinus koraiensis Siebold. et Zucc. cone water extracts and 80% ethanol extracts showed 40% or higher NO production inhibition compared to LPS treated control group. Determining expression of iNOS protein in cytoplasm with Western blot, un-ripening Pinus koraiensis Siebold. et Zucc. cone 80% ethanol extracts showed 50% or higher inhibition of iNOS protein expression. About 60% inhibition rate of COX-2, a producing factor of Prostaglandin E2, at 80% ethanol extracts 25μg/mL phenolic treated group. PGE2 25μg/mL treated water extracts and 80% ethanol extracts showed each 15% and 60% inhibition rate. Macrophage and TNF-α secreted from mast cell, inhibition rate were 20% with water extracts and 45% with 80% ethanol extracts of both 25μg/mL treated group. Un-ripening Pinus koraiensis Siebold. et Zucc. cone 80% ethanol extract 25μg/mL treated group showed 30% inhibition rate on IL-1β and 25% inhibition rate on IL-6. Therefore, Pinus koraiensis Siebold. et Zucc. cone extracts are very excellent substances for making to use industry of functional materials.

Key words: Anti-inflammatory, extracts, un-ripening Pinus koraiensis, Raw 264.7 Cell, lipopolysaccharide.