Antioxidant Effects of Humulus japonicus Extracts

Jung Woo Chae1, Hui Seon Jo1, Jin Young Lee2, Seung Jae Lee3, and Sung Hyun Joo4*
1Gyeonggido Forest Environment Research Center, Osan 447-290, Korea, 2Department of Herbal Cosmetics Science, Hoseo University, Asan 336-795, Korea, 3Aroma Newtech Co., Asan 336-120, Korea., 4Department of Forestry, Kyungpook University, Daegu 702-701, Korea

Humulus japonicus(HJ) is an annual plant and a vine plant. It is distributed mainly in Korea and East Asia, and it is mainly used for skin diseases in Korean folk remedies. HJ are killed by winding other plants with vines and have strong fertility. Therefore, it is classified as a harmful plant which is disturbing the ecosystem. To make the utilization, one of the methods for removing this plant, the antioxidant capacity of HJ was verified.  Experiment sample was collected in Mulhyanggi arboretum in june and august 2016, and extracted by 70% ethanol and hot water. After that, electron donating ability, ABTS radical scavenging activity and SOD-like activity was determinated. In all the experiments, if extract concentration increased, it increased activities. In electron donating experiment, electron donating ability of ethanol extracts is higher than hot water extracts, and june-harvested extract is higher than august-harvested extract. In ABTS experiment, ABTS radical scavenging activity of june-harvested ethanol extract is higher than august-harvested ethanol extract. The scavenging activity of june-harvested and august-harvested hot-water extracts were 67.16% and 82.1% at 1000 μg/ml concentration. As a result of SOD-like experiments, june-harvested ethanol extract was more active than august-harvested ethanol extract at high concentration than 100 μg/ml. The hot-water extracts showed no activity.

Key Word : Humulus japonicus, Anti-oxidant effect, Electron donating ability, SOD-like activity, ABTS radical scavenging activity

Supported by grants from KRF